返回产品列表
ProteanFect™ Max Mouse Immunocyte Transfection Kit
ProteanFect™ Max Mouse Immunocyte Transfection Kit is designed to efficiently transfect mouse primary immune cells, including T cells and NK cells. It achieves high transfection efficiency while maintaining minimal toxicity for these fragile immune cells. For research use only.
| 产品货号 | 规格（试剂B） | 价格（CNY） | 库存 |
|---|---|---|---|
| PT03-0002B | 0.02 mL | Inquire | Inquire |
| PT03-0010B | 0.1 mL | Inquire | Inquire |
| PT03-0100B | 1.0 mL | Inquire | Inquire |
转染性能
发表
Zhu, P. et al. Efficient Generation of CAR-T By the First Coacervate-Based Gene Delivery System. ASH Annual Meeting & Exposition, (2024).
Gao, X. et al. The First Coacervate-based Delivery System for Advanced Gene Therapy. ASGCT 28th Annual Meeting Abstracts, (2025).
Gao, X. et al. The First Coacervate-Based Delivery System for CAR-T Cell Engineering and Manufacturing. Presented at ASGCT 28th Annual Meeting Abstracts.
产品信息
试剂盒成分说明与储存方式
The kit is shipped on dry ice. Once received, store the components as indicated below. The kit includes positive control samples with EGFP-encoding mRNA to verify transfection efficiency.
| 成分说明 | 存储方式 |
|---|---|
| Reagent A | 2-8°C |
| Reagent B | -20°C |
| Reagent C | 2-8°C |
| EGFP mRNA (1 µg/µL) | -20°C |
运输方式
Shipped on Dry Ice
可转染细胞类型
Primary Cells Successfully Transfected Using ProteanFect™ Max Mouse Immunocyte Transfection Kit
| 细胞系 | 用于转染的兼容核酸 |
|---|---|
| Mouse primary T cells | mRNA, siRNA |
| Mouse primary NK cells | mRNA, siRNA |
| Mouse primary γδ T cells | mRNA, siRNA |
| Mouse primary Treg cells | mRNA, siRNA |
| Mouse primary CD4+ cells | mRNA, siRNA |
| Mouse primary CD8+ cells | mRNA, siRNA |
实验操作
实验操作步骤表（以96孔板转染mRNA为例）
| 步骤图 | 步骤 | Suspension Transfection |
|---|---|---|
 |
1. Transfection Complex Preparation |
1.1 Mix nucleic acids with Reagent A 1.2 Add Reagent B, and mix gently by pipetting up and down 20-30 times or vortexing for 10 seconds. 1.3 Add Reagent C. Mix gently by pipetting up and down 2-3 times or vortexing for 2-3 seconds. |
 |
2. Cell Preparation |
2.1 Harvest the cells by centrifugation at 300 g for 5 minutes. 2.1 Discard the supernatant and wash once with Opti-MEM. 2.3 Resuspend in Opti-MEM and adjust the concentration to 1×10⁷-1.5×10⁷ cells/mL. |
 |
3. Transfection |
3.1 Mix the transfection complex with the cell suspension and gently pipet up and down 2-3 times. 3.2 Incubate for 15-30 minutes in a cell culture incubator. 3.3 Add 10× volume of culture medium relative to the cell suspension. |
 |
4. Cell Culture and Test |
4.1 Transfer the cells from the tube to the culture plate. 4.2 Incubate transfected cells and assess efficiency after 5 to 48 hours, or at an appropriate time. |
| 步骤图 | 步骤 | Adherent Transfection |
|---|---|---|
 |
1. Cell Preparation |
1.1 Seed cells one day before transfection. |
 |
2. Transfectoin Complex Preparation |
2.1 Mix nucleic acids with Reagent A 2.2 Add Reagent B, and mix gently by pipetting up and down 20-30 times or vortexing for 10 seconds. 2.3 Add Reagent C. Mix gently by pipetting up and down 2-3 times or vortexing for 2-3 seconds. |
 |
3. Transfection |
3.1 Maintain 50%-80% cell confluence before transfection. 3.2 Remove medium, wash cells once with Opti-MEM, then add Opti-MEM. 3.3 Apply the transfection complex directly to the seeded cells. 3.4 Incubate for 15-30 minutes in a cell culture incubator. |
 |
4. Cell Culture and Test |
4.1 Replenish with the cell culture medium. 4.2 Incubate transfected cells assess efficiency after 5 to 48 hours, or at an appropriate time. |
常见问题解答
1.1 Optimize Transfection Parameters
Optimize transfection parameters for each cell type. Extended incubation time: Adjust the incubation time of the transfection complex with cells. The maximum incubation time is 30 minutes for primary cells. Increase transfection complex: Consider increasing the amount of transfection complex to improve transfection efficiency.
1.2 Severe Cytotoxicity Caused by Plasmid DNA
The transfection of pDNA into primary cells, such as primary T cells, can induce cytotoxicity and inflammatory responses. Due to the risk of significant toxicity, pDNA transfection is generally not recommended for primary T cells.
1.3 Improve Cell Condition
For primary mouse immunocytes, proper activation is crucial for optimal transfection efficiency. For example, mouse primary T cells generally achieve the best transfection results after stimulation with anti-CD3/CD28 activation beads or antibodies, with peak efficiency typically observed around days 2-3. Do not remove the activation beads or antibodies until transfection.
1.4 Use Positive Control
We recommend using a 96-well plate format to optimize transfection conditions for a specific cell type, with EGFP mRNA as the positive control.
Optimize transfection parameters for each cell type. Extended incubation time: Adjust the incubation time of the transfection complex with cells. The maximum incubation time is 30 minutes for primary cells. Increase transfection complex: Consider increasing the amount of transfection complex to improve transfection efficiency.
1.2 Severe Cytotoxicity Caused by Plasmid DNA
The transfection of pDNA into primary cells, such as primary T cells, can induce cytotoxicity and inflammatory responses. Due to the risk of significant toxicity, pDNA transfection is generally not recommended for primary T cells.
1.3 Improve Cell Condition
For primary mouse immunocytes, proper activation is crucial for optimal transfection efficiency. For example, mouse primary T cells generally achieve the best transfection results after stimulation with anti-CD3/CD28 activation beads or antibodies, with peak efficiency typically observed around days 2-3. Do not remove the activation beads or antibodies until transfection.
1.4 Use Positive Control
We recommend using a 96-well plate format to optimize transfection conditions for a specific cell type, with EGFP mRNA as the positive control.
Transfected cells may exhibit transient changes in behavior, but typically, viability will be restored by the second day post-transfection.
For further questions, please contact us at: tech@nanoportalbio.com.
Contacts
邮箱
contact@nanoportalbio.com
电话
订购客服
![]()